KLINICKÁ FARMAKOLOGIE A FARMACIE / Klin Farmakol Farm 2023;37(3):89-92 / www.klinickafarmakologie.cz 92 PŮVODNÍ PRÁCE Assay of colistin A, B and colistin methanesulfonate in human plasma by LC-MS/MS and short-term plasma stability components after 30 min. After 3 hours, the average conversion of CMS is about 13% at RT and 19% at 37 °C (Table 3). The stability of CMS in a matrix other than plasma was not tested. However, as there is evidence of lower stability of CMS in aqueous solutions or infusion solutions, this stability needs to be studied more closely before further measurement (7, 12). This analytical method for determining both COL and CMS in plasma samples is applied in the ongoing phase IV clinical trial „Pharmacokinetics of Colistin in Critically Ill Patients With Extracorporeal Membrane Oxygenation (COL-ECMO2022)” (EudraCT Number 2022-000291-19; NCT05542446) (13, 14). This study is designed to assess the influence of extracorporeal membrane oxygenation on the pharmacokinetics of colistin and CMS. Conclusion A rapid method for measuring colistin in human plasma has been introduced and validated. The described method is sensitive and selective for the analysis of colistin in plasma and it was applied to the measurement of patient samples. The presented paper is part of the Pharmacokinetics of Colistin in Critically Ill Patients With Extracorporeal Membrane Oxygenation (COL-ECMO2022) study in which further results will be presented. The study has been approved by the Ethics Committee of St. Anne’s University Hospital Brno (Number 10ML/2022-AM). EudraCT Number of the study is 2022-00029119, registered on June 21, 2022. The study was registered at the Clinical Trials register https://clinicaltrials.gov/ct2/show/NCT05542446 on September 15, 2022. Podpořeno ze státního rozpočtu prostřednictvím MŠMT projektem VVI CZECRIN (LM2023049) a IGA_LF_2023_004. REFERENCES 1. Rychlíčková J, Kubíčková V. Fyzikálně-chemické vlastnosti kolistinu a jejich dopady do klinické praxe. Klin Farmakol Farm. 2022;36(1):29-33. 2. Qi B, Gijsen M, Van Brantegem P, et al. Quantitative determination of colistin A/B and colistin methanesulfonate in biological samples using hydrophilic interaction chromatography tandem mass spectrometry. Drug Test Anal. 2020;12(8):1183-1195. 3. Rychlíčková J, Kubíčková V. Farmakologické vlastnosti kolistinu. Klin Mikrobiol Inf Lék. 2022;28(2):59-64. 4. Gikas E, Bazoti FN, Katsimardou M, at al. Determination of colistin A and colistin B in human plasma by UPLC–ESI high resolution tandem MS: Application to a pharmacokinetic study. J Pharm Biomed Anal. 2013;83:228-236. 5. Mercier T, Tissot F, Gardiol C, et al. High-throughput hydrophilic interaction chromatography coupled to tandem mass spectrometry for the optimized quantification of the anti- -Gram-negatives antibiotic colistin A/B and its pro-drug colistimethate. J Chromatogr A. 2014;1369:52-53. 6. Gobin P, Lemaitre F, Marchand S, Couet W, et al. Assay of Colistin and Colistin Methanesulfonate in Plasma and Urine by Liquid Chromatography-Tandem Mass Spectrometry. Antimicrob Agents Chemother. 2010;54(5):1941-1948. 7. Jansson B, Karvanen M, Cars O, et al. Quantitative analysis of colistin A and colistin B in plasma and culture medium using a simple precipitation step followed by LC/MS/MS. J Pharm Biomed Anal. 2009;49(3):760-767. 8. Zabidi MS, Abu Bakar R, Musa N, et al. Analytical methodologies for measuring colistin levels in pharmacokinetic studies J. Liq. Chromatogr. Relat. Technol. 2020;43(15-16):671-686. 9. Dagla I, Karkoula E, Baira E, et al. Analytical methodologies used for the determination of colistin in biological fluids. Is it still a challenge? J Pharm Biomed Anal. 2019;164:777-788. 10. Karvanen M, Malmberg C, Lagerbäck P, et al. Colistin Is Extensively Lost during Standard In Vitro Experimental Conditions. Antimicrob Agents Chemother. 2017;61(11):e00857-17. 11. Certificate of Analysis – Colistin sulfate (version LRAC9149.02). Merck (Sigma-Aldrich) [online]. [cit. 19-07-2023]. https://www.sigmaaldrich.com/CZ/en/product/sial/phr1605. 12. Mercier T, Tissot F, Gardiol C, et al. High-throughput hydrophilic interaction chromatography coupled to tandem mass spectrometry for the optimized quantification of the anti-Gram-negatives antibiotic colistin A/B and its prodrug colistimethate. J Chromatogr A. 2014;1369:52-63. 13. Pharmacokinetics of Colistin in Critically Ill Patients With Extracorporeal Membrane Oxygenation (COL-ECMO2022). ClinicalTrials.gov identifier: NCT05542446. Updated 28.12.2022 [online]. [cit. 19.7.2023]. https://clinicaltrials.gov/study/NCT05542446. 14. Suk P, Rychlíčková J, Součková L, et al. Changes of colistin pharmacokinetics in critically ill patients due to the extracorporeal membrane oxygenation: protocol for the COL- -ECMO2022 trial – a prospective, non-randomised, open- -label phase IV pharmacokinetic clinical trial. BMJ Open. 2023;13(7):e071649.
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