Klin Farmakol Farm. 2022;36(1):4-7 | DOI: 10.36290/far.2022.001
Determination of ampicillin plasma concentrations in single-dose administration for cardiac surgery prophylaxis
- 1 Ústav farmakologie LF UP a FN Olomouc
- 2 Kardiochirurgická klinika LF UP a FN Olomouc
The aim of this study was to determine the plasma concentration of ampicillin in plasma of patients undergoing myocardial revascularization without extracorporeal circulation and to calculate its basic pharmacokinetic parameters. An HPLC method with UV detection and Luna® Omega Polar C18 Column (250 × 4 mm; 5 µm) utilization was introduced and validated to determine ampicillin concentration. Individual pharmacokinetic parameters were calculated in MW Pharm 3.30 using a one-compartment model. Plasma concentrations of ampicillin were measured in 20 patients at 15, 30, 45, 60, 120, and 180 min after administration and at the end of surgery. Mean Cmax was found to be 127 ± 49 mg/l, elimination half-life 77 ± 29 min, total clearance 191 ± 73 ml/min, and distribution volume 19 ± 4 l. The calculated mean time for the plasma concentration of ampicillin to decrease to the predicted threshold of efficacy (mg/l) was 8.5 ± 3.5 h.
Keywords: ampicillin, prophylaxis, cardiac surgery, plasma concentrations.
Published: May 5, 2022 Show citation
References
- Onita T, Ikawa K, Nakamura K, Nishikawa G, Kobayashi I, Ishihara N, Tamaki H, Yano T, Naora K, Morikawa N. Prostatic Pharmacokinetic/Pharmacodynamic Evaluation of Ampicillin-Sulbactam for Bacterial Prostatitis and Preoperative Prophylaxis. J Clin Pharmacol. 2021;61:820-831.
Go to original source... 
Go to PubMed... - Mai XL, Phamb TV, Han GH, Kum SJ, Woo SH, Kang JS, Woo MH, Na DH, Chun IK, Kim KH. Simultaneous determination of ampicillin sodium and sulbactam sodium in powder for injection by HPLC. Anal Sci Technol. 2019;32:147-154.
- SPC Unasyn, Summary of Product Characteristics: https:// www.sukl.cz/modules/medication/detail.php?kod =0016600(accessed on Oct. 05, 2021).
- Grayson ML, Cosgrove S, Crowe SM et al. Kucers' the use of antibiotics: A clinical review of antibacterial, antifungal, antiparasitic, and antiviral drugs, seventh edition. Boca Raton: CRC Press, 2018. ISBN 9781498747950. Go to original source...
- Engelman R, Shahian D, Shemin R, Guy TS, Bratzler D et al. The Society of Thoracic Surgeons practice guideline series: Antibiotic prophylaxis in cardiac surgery, part II: Antibiotic choice. Ann Thorac Surg. 2007;83(4):1569-1576. Go to original source... Go to PubMed...
- do Nascimento TG, Aragao CFS, de Medeiros FD, Oliveira ED, Macedo RO. Validation of a method for determination of ampicillin in human plasma using LC-DAD. J Chrom Sci. 2009;47:749-755. Go to original source... Go to PubMed...
- Peng L, Wang X, Dang H. Simultaneous determination of meropenem and imipenem in rat plasma by LC-MS/MS and its application to a pharmacokinetic study. Biomed Chromatogr. 2021;35(11):e5185. Go to original source... Go to PubMed...
- Wildfeuer A, Müller V, Springsklee M, Sonntag HG. Pharmacokinetics of ampicillin and sulbactam in patients undergoing heart surgery. Antimicrob Agents Chemother. 1991;35(9):1772-1776. Go to original source... Go to PubMed...