Klin Farmakol Farm. 2013;27(3-4):101-105

Effect of Pseudomonas aeruginosa resistance on antibiotic therapy

Milan Kolář, Miroslava Htoutou Sedláková, Markéta Šenkyříková, Vojtěch Hanulík, Pavel Honig

Ústav mikrobiologie, LF UP a FN Olomouc

Pseudomonas aeruginosa strains are among very important bacterial pathogens, particularly in the case of nosocomial infections. The frequency

of this species as a causative agent of infections, including life-threatening ones, is increasing, and so is its resistance to the effect

of antibiotic therapy. The aim of the present paper was to determine the resistance of Pseudomonas aeruginosa to antibiotics and to analyze

the group of meropenem-resistant isolates. In the period from 1 January 2008 to 30 June 2013, a total of 11,342 strains of Pseudomonas

aeruginosa were isolated from the clinical material of patients hospitalized in the Olomouc University Hospital and another 1,341 strains

were isolated in the case of patients from the Olomouc Military Hospital. Resistance to antimicrobial agents was assessed using the standard

dilution micromethod. In 57 meropenem-resistant strains of Pseudomonas aeruginosa, the production of metallo-beta-lactamases, serine

carbapenemases, and AmpC enzymes was determined by means of both phenotype and genotype methods. An increasing frequency of

Pseudomonas aeruginosa strains resistant to meropenem and piperacillin with tazobactam was demonstrated. In a number of isolates,

sensitivity only to colistin and amikacin remained preserved. The production of metallo-beta-lactamases and serin carbapenemases was

not confirmed, and resistance to meropenem is particularly related to the production of AmpC and the loss of the cell wall porins.

Keywords: Pseudomonas aeruginosa, infection, treatment

Published: December 1, 2013  Show citation