Klin Farmakol Farm. 2021;35(1):24-28

Analytical methods for the determination of cefazolin

Vendula Kubíčková, Zuzana Rácová
Ústav farmakologie LF UP v Olomouci

Cefazolin is a broad-spectrum antibiotic used primarily as a prophylactic care for patients during surgery. In specific groups of patients, we encounter variability in pharmacokinetics from the population average. To optimize dosing regimens, reliable bioanalytical methods capable of rapid sample determination should be used. Currently, the most widely used method for measuring plasma concentrations is liquid chromatography with UV or MS detection. Protein precipitation and solid phase extraction can be used for pre-analytical sample preparation. Equilibrium dialysis or ultrafiltration can be used if the free fraction needs to be determined. Undesirable factors affecting the accuracy of the assay may be matrix effects or low sample stability.

Keywords: cefazolin, HPLC, LC-MS, free and bound drug fraction.

Published: April 9, 2021  Show citation

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Kubíčková V, Rácová Z. Analytical methods for the determination of cefazolin. Klin Farmakol Farm. 2021;35(1):24-28.
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