Klin Farmakol Farm. 2010;24(3):121-123
Determination of imipenem plasmatic concentrations by high-performance liquid chromatography
- 1 Ústav farmakologie LF UP a FN Olomouc
- 2 Klinika anesteziologie, resuscitace a intenzivní medicíny, Všeobecná fakultní nemocnice Praha
Imipenem is a high-effective broad-spectrum carbapenem antibiotic. The aim of our study was to introduce a reliable and simple
method for determination of imipenem plasma concentrations in our scientific laboratory. Plasma samples were stabilized by MOPS.
Elution was performed in gradient mode, mobile phase composed of potassium dihydrogenphosphate and methanol. Imipenem was
detected at 313 nm, no intereference of cilastatin or thienamycin was found. The basic validation parameters of method presented here
have been also determined.
Keywords: imipenem, high-performance liquid chromatography, pharmacokinetics, therapeutic monitoring
Published: October 15, 2010 Show citation
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